Figure 1 from Controlled hot start and improved specificity in carrying out PCR utilizing touch-up and loop incorporated primers (TULIPS). | Semantic Scholar
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SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly on length and composition of the primer(s). An annealing temperature 5°C
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Calculating the melting temperature of PCR primers
